spectramax i3x (Danaher Inc)The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. 312 gain calibration failed. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The MPO assay was conducted according to the. Find regional support. The assay window spanned four orders of magnitude. Table 1. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Multimode Reader Comparison Chart. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 3: The cuvette chamber. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesThe SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. We would like to show you a description here but the site won’t allow us. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Increased fluorescence performance. Danaher Inc spectramax i3x. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Prime function 20 amol. Description 1. no. ATP (“flash” luminescence using . A mixture of 6-nitro-1H,3H-benzo[de]isochromene-1,3-dione (486 mg, 2. 0. In addition, Image J was used to quantify the. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. 314 low lamp level warning. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. The SoftMax Pro Software provides integrated instrument control. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. Nuclear Receptor. Download PDF. 10, and 0. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). Figure 1. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. Introduction. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. . Replicate values were averaged and imported into Design-Expert 8. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. As. See Unpacking the SpectraMax Paradigm Instrument on page 1. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 7 mm for all shake intensities. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Register to download your free eBook today. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Bioz Stars score: 98/100, based on 1 PubMed citations. Learn how the SpectraMax Paradigm Multi-Mode Detection Platform with Tunable Wavelength (TUNE) Detection Cartridge combines the flexibility of wavelength scanning with the high sensitivity of filters. 12 mg/mL) of polysaccharides were prepared after addition of glucosum anhydricum for calibration. Specifically, we present neurite. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. Figure 4. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. User-upgradeable detection modules allow you to expand the capabilities. HTRF-compatible SpectraMax Multi-Mode Readers. Synthesis of NORP Synthesis of compound 1. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. *On the SpectraMax i3x reader only. In this product video, see how the SpectraMax M5 quickly reads samples. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. 2. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe absorbance was recorded on a spectra Max i3x microplate reader (Molecular Devices, USA) at 450 nm. 316 grating motor driver faulty. 11. See SpectraMax® Microplate Reader User Manual Download Page. The LDH activity in the cultural media was measured based on the reaction between LDH and lactic acid, which led to the generation of pyruvic acid, and pyruvic acid could become brown in alkaline environments with 2,4. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. 200-800 nm using Spectra Max i3X microplate reader. 635. In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. This is not due to a temperature-dependent alteration in cellular autofluorescence,. The SpectraMax i3 is specifically used in this lab for BCA assays and Elisa Assays. Patented user-exchangeable cartridge design expands the system's detection capability making it. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. 2C). 2. Example: [Drawer]. each new assay. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 02, 0. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. It also integrates with the. Increased fluorescence performance. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. Molecular Devicesの全商品を見る. Modularity does provide some measure of forward vision into upcoming or future experimental needs. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Spectramax I3x, supplied by Danaher Inc, used in various techniques. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. Microplate readers, multi-mode, SpectraMax® i3x. Order Now. SpectraMax i3x and Paradigm readers require the FP-FLUO. Capture Flash Assays with Ease. Multimode Readers Comparison Chart. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. The SpectraMax i3x reader provides theAbsorption was measured at 490 nm by utilizing a Spectra max i3x Multimode microplate reader (Molecular Devices). 4. Settings not required by a reader are indicated by ‘---’. ISM Equipment Classification (Group 1, Class A) This equipment is. SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. D 1 1. Multimode Reader Comparison Chart. com Check our website for a current listing of worldwide distributors. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Immunofluorescence StainingAfter cooled down to room temperature, the concentrations of the solution were measured by microplate reader (Spectra Max i3x) at 490 nm (Kagi and Schaffer, 1988). 발광 (Luminescence) 리더기. The treatments were. • Removing the transport locks from the PMT shutters and drawers. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . 99（图一）。After incubation at room temperature for one hour in the dark, we measured the fluorescence intensity using a SpectraMax i3x (Molecular Devices, CA, USA), with excitation at 350 nm and dual emissions at 435 nm and 490 nm. i3x. ATP (“flash” luminescence using . The SoftMax Pro Software provides integrated instrument control. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The CellTiter-Glo assay from Promega uses luciferase. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. It allows the user to determine the total concentration of protein in a solution. Examples: “Press carefully to engage,” “ Do not. Our patent-pending StainFree Cell Detection Algorithm enables cell counting and confluency measurements. UV-Vis absorbance, fluorescence and luminescence • Accommodates plate types from six to 384-well format • Easily upgradeable with interchangeable filter cubesSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. 00800 665 32860. 0´ 104 4. Bioz Stars score: 98/100, based on 1 PubMed citations. We would like to show you a description here but the site won’t allow us. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalSpectraMax i3x Multi-Mode Microplate Reader. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. ZERO BIAS - scores, article reviews, protocol conditions and moreWe would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us. User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Italic and boldface type are used for emphasis. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. 흡광 (Absorbance) 리더기. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The SpectraMax ® i3x. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. The plate was shaken and incubated in the dark for 10 minutes. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. applications, including dual luciferase and ATP assays. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The FRET ratio is defined as the relative fluorescence intensity of an acceptor normalized to the total fluorescence intensity. 660-665 Eskdale Road. 1. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. ; The top 3 importers of Spectramax are India with 365. Simplified workflow: Add cellular imaging assays in a small footprint. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Check software compatibility with Windows 10 and locate your software installer. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax Microplate Readers. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. iD5. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. , Fisher Scienti fi c, cat. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a 96-well black, clear-bottom microplate. Microplate Stacker. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. Prime function 20 amol. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. ) wide and 65 cm (25. with best-in-class data analysis software • Three modes of detection for . **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. It allows you to explore cellular . Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Open the catalog to page 1. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. Sunnyvale, CA, USA) with excitation at 475 nm. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. Fluorescence Microplate Reader Comparison Chart. This instrument also provides the option of modular upgrades for Western Blotting,. Explore a wealth of applications with the future-ready SpectraMax i3x. Data Analysis. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. SpectraMax i3, i3x, and iD5 readers. SpectraMax ® iD3 and iD5 Multi-Mode . 6% for the on-board optics (monochromators). Microplate reader assays requiring infrared (IR) or red-shifted. 제품. Pick the best speed, sensitivity, and price for your needs. Eliminate time-dependent substrate addition steps. Every SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Bookings are free, but you will need to use iLab to book. The fluorescence spectra were acquired using a microplate reader (Spectra Max i3x; Molecular Devices, USA). 0001 0. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 7°C (gradient heating design). The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Find regional support. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. Price from $9. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. 価格、見積もり、お近くの販売店を. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Quickly and easily expand the capabilities of your SpectraMax Paradigm or i3x Multi-Mode Microplate reader to include Tunable Wavelength detection using the. We would like to show you a description here but the site won’t allow us. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). Description 1. SpectraMax i3x. 2 only) SpectraMax M-series Microplate readers. SpectraMax i3x 一套系统即可以帮助你通过科学手段解开细胞通路的奥秘，也可以检测并分析出 样品中蛋白质的活性和表达情况。SpectraMax i3x 检测系统可以根据未来实验需求，无需售后工 程师协助即可加入全新检测功能模块，真正做到一次投入终身受益。1) Select the correct plate and the wells that you want to measure. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. The dynamic range of the assay is about 3. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. 2 (Molecular Devices, San Jose, CA). The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Prepare your i3/i3x instrument for shipment. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 1. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 315 can't find zero order. 7. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Published Dec 16, 2020 | Updated Dec 16, 2020. 2 only) SpectraMax M-series Microplate readers. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax M 시리즈 Microplate Reader, Cuvette Port, IQ OQ 검증 | Molecular Devices. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. However, no piece of equipment is immune to breaking down. Singlet oxygen (1 O 2) generation was determined through 1,3-diphenylisobenzofuran (DPBF) quenching. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 1. ZERO BIAS - scores, article reviews, protocol conditions and more. Time-. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. , 2014; Xiong et al. Detection of pro-inflammatory factors. Methods. Molecular Devices are known for both the reliability and the wide uses of their products. Description 1. 0. Prepare your iD series instrument for shipment. Materials and methodsWe would like to show you a description here but the site won’t allow us. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Also refer to this Multimode Reader Comparison Chart. 22 μ m fi ltration unit (Sigma, cat. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 9 x 10-15. Molecular Devices Corporation. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Art-science interactive light show/animation. Carbonyl assay The plates for carbonyl detection were prepared as described. 3: The cuvette chamber. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. 3. Promega. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. 306 plate air cal. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected. Find regional support. Spectranet customer portalThe Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). Multimode Reader Comparison Chart. 2 in. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. It allows you to explore cellular pathways and protein activation and expression in one system. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. china@moldev. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Increased fluorescence performance. It features a modular design that allows users to add or change detection cartridges according to their needs. +44 118 944 8000. no. 0´ 104 6. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Patented user-exchangeable cartridge design expands the system's detection capability making it. Recommended settings for SpectraMax iD5 and i3x readers. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1).